proliferate resonse to purified bordetella pertussis toxin on murine spleen lymphocytes

نویسندگان

a. pourahmadi

f. esmaili

a. zavaran hosseini

a. rezaee

e. salehi

چکیده

a purification procedure of pertussis toxin (pt) from submerged culture of bordetella pertussis (b.pertussis) strain 134 using adsorption and affinity chromatography was discussed. the yield of the resulting pt was approximately 37.5mg/l of concentrated culture supernatant. the polypeptide pattern of the purified pt was investigated by sds-page and showed five bands between 11 to 26 kda using low molecular weight marker. since pt is the main component of acellular pertussis vaccine, obtaining a good yield of it would be essential for production of the new and safer vaccine generation. the other objective of this study was to determine the effects of pt on murine lymphocytes using mtt test. the effect of various doses of prepared pt on murine lymphocytes showed that the amount of 0.5μg/0.1ml had the highest proliferation. furthermore comparison between the resulting pt and phytohemagglutinin showed much higher effect of pt on murine spleen cells. these results indicate that b.pertussis strain 134 is a suitable strain for induction of pt in order to use it for development of acellular vaccine in iran and also for in vitro studies on proliferation of murine lymphocytes.

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Proliferate Resonse to Purified Bordetella pertussis Toxin on Murine Spleen Lymphocytes

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عنوان ژورنال:
archives of razi institute

ناشر: razi vaccine & serum research institute (rvsri)

ISSN 0365-3439

دوره 58

شماره 1 2016

میزبانی شده توسط پلتفرم ابری doprax.com

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